High Efficient Reverse Transcriptase: ReverTra Ace®
ReverTra Ace® is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace® has two mutations in the polymerase and RNase H domains that elevate the cDNA synthesis ability and inactivate RNase H activity, respectively.Therefore, ReverTra Ace® (Code No. TRT-101) exhibits efficient reverse transcriptase activity and enables the synthesis of ≥ 14 kb cDNA.
ReverTra Ace-α-® (Code No.FSK-101) is an efficient and convenient kit for synthesizing high quality cDNA. The kit contains the highly efficient reverse transcriptase "ReverTra Ace®" as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.
Fig.1 Point mutation in M-MLV RTase
- ReverTra Ace
- Reverse Transcriptase (Company A)
- Reverse Transcriptase (Company B)
Fig.2 Comparison of reverse transcriptase efficiency.
Reverse transcriptase efficiency among various RTases is compared by RT-PCR of 18S rRNA. The RTase reaction was performed using a specific primer at 42°C.