Life Science

PCR Highly Functional PCR Enzyme

Tth DNA polymerase is a family A DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8 (1). This polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ions. Therefore, this enzyme enables "one-step RT-PCR" including reverse transcription and PCR steps. Moreover, this enzyme exhibits 5'→3' exonuclease activity that can be used in the TaqMan® assay in real-time PCR.

References

1. T. W. Myers and D. H. Gelfand, Biochemistry, 30: 7661-6 (1991)

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